Technical Data
BLAME (B-lymphocyte Activator Macrophage Expressed)
B Lymphocyte Activator Macrophage-Expressed (BLAME), also known as SLAMF8, is an Ig superfamily protein that is part of the CD2 family of receptors. It is expressed on macrophages and dendritic cells. BLAME modulates signaling through the B-cell receptor and may also function during B-lineage commitment.

Suitable for use in Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 25ug/ml. Use 10ul to label 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul.
Direct ELISA: 0.5-1.0ug/ml. Detection limit is ~5ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20CBlue IceHumanMouse
NS0-derived recombinant human BLAME extracellular domain (aa23-233; Accession # Q9P0V8)
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human BLAME. Does not react with recombinant human SLAMF7 or recombinant human SLAMF6.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.