Technical Data
B2106-70C
Blimp-1 (BLIMP 1, BLIMP1, B Lymphocyte Induced Maturation Protein 1, Positive Regulatory Domain I Binding Factor 1, PR Domain Containing 1, PRDI BF1, PRDM1)
Description:
Human BLIMP1(B lymphocyte­induced maturation protein 1, also known as PRDM1)is a 91kD zinc­finger transcriptional repressor that promotes B cell maturation into plasma cells. It is 789aa in length, and contains an N­terminal S­E­T domain (aa64­170) and our C­terminal C2H2­t ype zinc­finger motifs (aa 539­645). The SET domain interacts with chromatin modifers, while the zinc fingers bind to DNA. There is one 80kD 691aa alternate splice form that utilizes an
intenal start site. This results in a substitution of three aa for the first 101aa of the long form, and the loss of the SET domain. At least 10 mutations exist, resulting in proteins of 61­603aa in length. Over aa667­789, human BLIMP1 is 89% aa identical to mouse BLIMP1.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1mg/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG11D96Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa667-789 from human Blimp-1 expressed in E. coli (O75626).
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human Blimp-1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.