Technical Data
B2106-70D
BLIMP1 (PR Domain Zinc Finger Protein 1, BLIMP-1, Beta-interferon Gene Positive Regulatory Domain I-binding Factor, PR Domain-containing Protein 1, Positive Regulatory Domain I-binding Factor 1, PRDI-BF1, PRDI-binding Factor 1, PRDM1)
Description:
BLIMP-1 is a 97kD multidomain transcription repressor that has a SET domain in its N-terminal region and four C2H2-type zinc fingers in its C-terminal regions. It promotes terminal differentiation of B cells into plasma cells and also has an important role in primordial germ cell specification. An alternative splice variant that lacks 101 N-terminal aa residues of full-length BLIMP1 has been reported. Over the region used as immunogen, human and mouse BLIMP1 share 89% aa sequence identity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
Not determined
Immunogen:
Purified, E. coli-derived, recombinant human B Lymphocyte-Induced Maturation Protein 1 (aa687-789; Accession # 075626).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes human BLIMP1. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.