		Technical Data
B2106-70D	BLIMP1 (PR Domain Zinc Finger Protein 1, BLIMP-1, Beta-interferon Gene Positive Regulatory Domain I-binding Factor, PR Domain-containing Protein 1, Positive Regulatory Domain I-binding Factor 1, PRDI-BF1, PRDI-binding Factor 1, PRDM1)

Description:
BLIMP-1 is a 97kD multidomain transcription repressor that has a SET domain in its N-terminal region and four C2H2-type zinc fingers in its C-terminal regions. It promotes terminal differentiation of B cells into plasma cells and also has an important role in primordial germ cell specification. An alternative splice variant that lacks 101 N-terminal aa residues of full-length BLIMP1 has been reported. Over the region used as immunogen, human and mouse BLIMP1 share 89% aa sequence identity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml
Western Blot: 0.1-0.2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

Not determined

Immunogen:

Purified, E. coli-derived, recombinant human B Lymphocyte-Induced Maturation Protein 1 (aa687-789; Accession # 075626).

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

Specificity:

Recognizes human BLIMP1. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.