Technical Data
B2106-70G
BLIMP1 (PR Domain Zinc Finger Protein 1, BLIMP-1, Beta-interferon Gene Positive Regulatory Domain I-binding Factor, PR Domain-containing Protein 1, Positive Regulatory Domain I-binding Factor 1, PRDI-BF1, PRDI-binding Factor 1, PRDM1)
Description:
PRDM1, an 88kD novel transcription repressor protein is a human homolog of mouse Blimp1. It belongs to the family of PRDM protein family and contains a PR domain and 5 Kruppel Like-zinc finger motifs at the C-terminal region. It plays a pivotal role in inducing and regulating terminal differentiation of B-lymphocytes. It recruits HDAC and complex of Groucho/TLE family proteins to the DNA and actively represses vital genes required for B-cell proliferation including c-myc, CIITA promoter III, CD23b and PAX-5 promoter. PRDM1 silences IFN-gene expression by binding specifically to PRDI of the IFN gene promoter. It is continuously expressed in all Plasma cells, but is not detected in early bone marrow B-cells, memory B-cells in spleen and most germinal center B-cells. In myeloma cells, PRDI-BF1, a truncated isoform of PRDM1 is expressed that has an impaired ability to repress transcription suggesting a clinical implication.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa185-199 of human PRDM1.
Purity:
Serum.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human BLIMP-1. Species sequence homology: chimpanzee.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Keller,A.D. and Maniatis,T. Identification and characterization of a novel repressor of beta-interferon gene expression. Genes Dev. 5 (5), 868-879 (1991). 2. Gyory,I., Fejer,G., Ghosh,N., Seto,E. and Wright,K.L. Identification of a functionally impaired positive regulatory domain I binding factor 1 transcription repressor in myeloma cell lines. J. Immunol. 170 (6), 3125-3133 (2003). 3. Jin Yu et.al Molecular and Cellular Biology, 20 (7); 2592-2603 (April 2000) 4. Shaffer AL et.al Immunity 17(1):51-62 (2002 Jul) 5. C. Tunyaplin et.al The Journal of Immunology, 173: 1158-1165 (2004) 6. Gyory. I et.al Nat Immunol 5(3):299-308 (2004) 7. Turner CA Jr. et.al Cell Apr 22; 77(2):297-306 (1994).