Technical Data
B2106-70J
BLIMP1 (PR Domain Zinc Finger Protein 1, BLIMP-1, Beta-interferon Gene Positive Regulatory Domain I-binding Factor, PR Domain-containing Protein 1, Positive Regulatory Domain I-binding Factor 1, PRDI-BF1, PRDI-binding Factor 1, PRDM1)
Description:
PRDM1, an 88KDa novel transcription repressor protein is a human homolog of mouse Blimp1. It belongs to the family of PRDM protein family and contains a PR domain and 5 Kruppel Like-zinc finger motifs at the C-terminal region. It plays a pivotal role in inducing and regulating terminal differentiation of B-lymphocytes. It recruits HDAC and complex of Groucho/TLE family proteins to the DNA and actively represses vital genes required for B-cell proliferation including c-myc, CIITA promoter III, CD23b and PAX-5 promoter. PRDM1 silences IFN-gene expression by binding specifically to PRDI of the IFN gene promoter. It is continuously expressed in all Plasma cells, but is not detected in early bone marrow B-cells, memory B-cells in spleen and most germinal center B-cells. In myeloma cells, PRDI-BF1, a truncated isoform of PRDM1 is expressed that has an impaired ability to repress transcription suggesting a clinical implication.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human Lymph Node lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to KVKQETVEPMDP, from the C-terminus of the protein sequence according to NP_001189.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.
Specificity:
Recognizes human BLIMP1. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chang DH, Angelin-Duclos C, Calame K.BLIMP-1: trigger for differentiation of myeloid lineage.Nat Immunol. 2000 Aug;1(2):169-76. PMID: 11248811. 2. Turner CA Jr. et.al Cell Apr 22; 77(2):297-306 (1994). 3. Jin Yu et.al Molecular and Cellular Biology, 20 (7); 2592-2603 (April 2000). 4. Shaffer AL et.al Immunity 17(1):51-62 (2002 Jul). 5. C. Tunyaplin et.al The Journal of Immunology, 173: 1158-1165 (2004). 6. Gyory. I et.al Nat Immunol 5(3):299-308 (2004).