Technical Data
BLIMP1, NT (PR Domain Zinc Finger Protein 1, BLIMP-1, Beta-interferon Gene Positive Regulatory Domain I-binding Factor, PR Domain-containing Protein 1, Positive Regulatory Domain I-binding Factor 1, PRDI-BF1, PRDI-binding Factor 1, PRDM1)
Blimp-1 was initially identified as a zinc finger-containing protein that drives the maturation of B lymphocytes into immunoglobulin-secreting cells. Together with X-box- binding protein 1 (XBP1), Blimp-1 is induced upon terminal differentiation of plasma cells. The transcriptional repressor activity of Blimp-1 has also been found to regulate T cell homeostasis and function, possibly by suppressing the expression of the cytokines IL-2 and interferon-g during T cell development. More recent experiments have suggested that Blimp-1 also plays a major role in the formation of primordial germ cells (PGC) in developing mammalian embryos. In these experiments, Blimp-1-deficient mutant mouse embryos form a cluster of PGC-like cells which fail to show the expected migration, proliferation, and repression of homeobox genes that normally accompany specification of primordial germ cells.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Mouse lung tissue lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to 17aa from human Blimp-1, at N-terminal.
Supplied as a liquid in PBS, 0.02% sodium azide.
Recognizes human Blimp-1. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Turner CAJ, et al (1994) Cell. 77:297-306. 2. Angelin-Duclos C, et al (2000) J. Immunol. 165:5462-71. 3. Reimold AM, et al (2001) Nature. 412:300-7. 4. Martins GA, et al (2006) Nature Immunol. 7:457-65. 5. Ohinata Y, et al (2005) Nature. 436:207-13.