Cellular Localization: Cell Membrane
Suitable for use in ELISA and Immunoprecipitation. Other applications not tested.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|100ug||4°C (-20°C Glycerol)||Blue Ice||Human||Mouse|
|Cell preparation: A431 cells (human vulval squamous carcinoma cell line).|
|Supplied as a liquid in PBS, pH 7.2. No preservative added.|
|Suitable for binding to the EGF receptor in A431 cells only. These cells have a glycosylation defect which reveals otherwise cryptic sugar structures. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
This product has been used in:Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). <2266467>Parker PJ et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984). <6086658>|