Technical Data
B2125-07
Blood Group A
Description:
Cellular Localization: Cell Membrane

Applications:
Suitable for use in ELISA and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG38J337Purified
SizeStorageShippingSourceHost
100ug4C Do Not FreezeBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Cell preparation: A431 cells (human vulval squamous carcinoma cell line).
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Suitable for binding to the EGF receptor in A431 cells only. These cells have a glycosylation defect which reveals otherwise cryptic sugar structures. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). Parker PJ et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984).