Description:
Cellular Localization: Cell Membrane
Applications:
Suitable for use in ELISA and Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG3 | 8J337 | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | 4°C (-20°C Glycerol) | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Cell preparation: A431 cells (human vulval squamous carcinoma cell line). |
| Purity: |
| Purified |
| Form |
| Supplied as a liquid in PBS, pH 7.2. No preservative added. |
| Specificity: |
| Suitable for binding to the EGF receptor in A431 cells only. These cells have a glycosylation defect which reveals otherwise cryptic sugar structures. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
This product has been used in:Gullick WJ & Lemoine NR 'EGF receptor' antibody 29.1.1. J Pathol 162:275-6 (1990). Parker PJ et al. Monoclonal antibodies against the human epidermal growth factor receptor from A431 cells. Isolation, characterization, and use in the purification of active epidermal growth factor receptor. J Biol Chem 259:9906-12 (1984).
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