Technical Data
B2125-12
Blood Group AB
Description:
The ABO system is based on differential gylcosylation of lipid or protein on blood cells. The basic carbohydrate chain extending from the protein or lipid is, eg, Type O: Protein or Lipid - Glucose--N-Acetylglucosamine--Galactose--Fucose. The galactose residue can have an additional sugar residue (in addition to the Fucose) attached to it. If the additional residue is N-Acetylgalactosamine, then the individual is Type A. If the additional residue is Galactose, then the individual is Type B. Type A's and Type B's actually differ because they have different enzymes for glycosylation at the Galactose site. Type A: N-Acetylgalactosamine glycosylation; Type B: Galactose glycosylation. Type AB: individuals with both enzymes. (Type AB individuals have N-Acetylgalactosamine on some galactose sites and Galactose on other galactose sites. Type AB are a mixture of Type A and Type B blood.) Type O individuals do not have the enzymes for glycosylation on the galactose site.

Applications:
Suitabe for blood group typing in direct agglutination tests, slide test, tube test, microplate test, ELISA, Western Blot, Flow Cytometry, and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM1V017Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in 0.3M sodium chloride, 1.25mM EDTA, 0.1% sodium azide.
Specificity:
Recognizes AB human blood antigens. Weak crossreactivity with A1; must be diluted appropriately for automated tests.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.