Technical Data
B2125-14G
Blood Group B
Description:
Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Applications:
Suitable for use in Agglutingtion and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen, paraffin): 1:10-2000
Optimal dilutions to be determined by the researcher.

Titer:
Agglutingtion: 1:128 with erythrocytes of blood group B.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM8J342Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Mixture of erythrocytes of blood group B and glycoprotein fraction isolated from the saliva of secretors with blood group B.
Purity:
Supernatant
Form
Supplied as a liquid. Preservative free
Specificity:
Recognizes human blood group B antigens. The specifity of the antibody was confirmed by the comparison of specifity and reactivity to standard reagent using >5000 samples of blood.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Vanak J et al. (1989) Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma 36:479-88.