Description:
Fusion Partners: Spleen cells from immunized Balb/c were fused with cells of a myeloma cell line.
Recommended Negative Controls: Mouse IgG1 Negative Control
Applications:
Immunoprecipitation
Haemagglutination
Optimal working dilutions to be determined by researcher.
Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 3H571 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 200ug | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Human erythrocytes. |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Purified IgG - liquid in Tris buffered glycine, pH 8 with 0.1% sodium azide. |
| Specificity: |
| Recognizes the kell blood group antigen Kpbc. The kell blood group antigens are carried on a 93kD erythrocyte membrane glycoprotein. In addition clone BRIC203 will agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but does not react with Ko erythrocytes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Parsons, S.F. et al. (1992). Monoclonal antibodies against kell glycoprotein: serology, immunochemistry and quantification of antigen sites. Transfusion Medicine 3: 137-142.
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