Technical Data
B2125-28H
Blood Group Lewis y (FUT3, Lewis Y Blood Group)
Description:
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y and precursor type 1 chain antigens. Increased expression of Lewis B has been reported in gastric and pancreatic tumors as well as in colorectal cancer.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin and frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgMA70-C/C8Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.1mg/ml
Immunogen:
Live cells of the human colorectal carcinoma cell line Ls174T from a NZB/N Bom mouse.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes the epitope Fuc-a-1-2Gal-b-1-4(Fuc-a-1-3)GlcNAc-b-1 of the human blood group Lewis Y. Does not cross react with the blood group H type 2 antigens or with Lea, sialyl-Lea, Lex , sialyl-Lex, Leb or Lec.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.