Technical Data
B2553-03B
Bone Morphogenetic Protein 1, CUB2 Domain (BMP1, ProCollagen C-Endopeptidase, PCP, mTld)
Description:
BMP-1 is an extracellular zinc endopeptidase of the Astacin family, which also includes Astacin, Meprin-A and B, Tolloid-like proteins and Choriolysin. The name BMP-1 reflects the involvement of PCP in the collagen deposition of growing bone. Procollagen C-peptidase cleaves the carboxy end from procollagen, to allow formation of mature, triplehelical collagen. The Aminoterminal end must also be cleaved by the Procollagen N-Proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP-1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP-1 have been reported, with varying truncation at the carboxyterminal end. The long form of BMP-1 is most similar to the Tolloid-like proteins, which have extra EGF-like and CUB domains. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen.

Applications:
Western blot: Starting dilutions 1:1000 for colorimetric substrates such as BCIP/NBT, 1:5000 for chemiluminescence substrates.


Storage and Stability: The undiluted antibody solution is stable for approximately 12 months at 40C. For longer storage, store at-200C. The solution is buffered from freezing at -20C by 50% glycerol, but will freeze at-80C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 40C with 0.05% Sodium Azide as preservative.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
The antibody is made to a synthetic peptide based on the CUB-2 domain of the human sequence
Purity:
Peptide affinity purified.
Form
Supplied as a liquid in PBS buffer, pH 7.4, with the addition of 0.01% sodium azide as preservative and 50% glycerol as cryoprotectant.
Specificity:
Reactive with Bone Morphogenic protein-1 (BMP-1, PCP, ProCollagen CPeptidase). Species Crossreactivity: Human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.