Technical Data
B2553-03B
Bone Morphogenetic Protein 1, CUB2 Domain (BMP1, ProCollagen C-Endopeptidase, PCP, mTld)
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Technical Data |
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B2553-03B |
Bone Morphogenetic Protein 1, CUB2 Domain (BMP1, ProCollagen C-Endopeptidase, PCP, mTld) |
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Description: BMP-1 is an extracellular zinc endopeptidase of the Astacin family, which also includes Astacin, Meprin-A and B, Tolloid-like proteins and Choriolysin. The name BMP-1 reflects the involvement of PCP in the collagen deposition of growing bone. Procollagen C-peptidase cleaves the carboxy end from procollagen, to allow formation of mature, triplehelical collagen. The Aminoterminal end must also be cleaved by the Procollagen N-Proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP-1 are thought to lead to aberrant collagen processing, and connective tissue disorders. Many forms of BMP-1 have been reported, with varying truncation at the carboxyterminal end. The long form of BMP-1 is most similar to the Tolloid-like proteins, which have extra EGF-like and CUB domains. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Applications: Western blot: Starting dilutions 1:1000 for colorimetric substrates such as BCIP/NBT, 1:5000 for chemiluminescence substrates. Storage and Stability: The undiluted antibody solution is stable for approximately 12 months at 40C. For longer storage, store at-200C. The solution is buffered from freezing at -20°C by 50% glycerol, but will freeze at-80°C. Avoid unnecessary freeze-thaw cycles. Diluted antibody can be reused several times if not contaminated; store at 40C with 0.05% Sodium Azide as preservative. |
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