Technical Data
Bone Morphogenetic Protein 2 (BMP2), Human, Recombinant
Molecular Biology Storage: -70CShipping: RT
BMPs (Bone Morphogenetic Proteins) belong to the TGF-beta superfamily of structurally related signaling proteins. BMP-2 is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with osteoconductive carriers such as collagen and synthetic hydroxyapatite. In addition to its osteogenic activity, BMP-2 plays an important role in cardiac morphogenesis, and is expressed in a variety of tissues including lung, spleen, brain, liver, prostate ovary and small intestine. The functional form of BMP-2 is a 26kD protein composed of two identical 114 amino acid polypeptide chains linked by a single disulfide bond.

Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino acid signal sequence for secretion, and a 259 amino acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant human BMP-2 is a 26kD homodimeric protein consisting of two 115 amino acid polypeptide chains.

rhBMP-2 is expressed in E. coli and renatured from inclusion body material.

The currently know receprors for BMP-2 are integral membrane proteins. Signaing into th einterior of the cells proceeds upon ligand-induced receptor oligomerization that results in the activation of serine/threonine kinases.

Biological Activity:
BMP-2 induces alkaline phosphatase expression at an EC50 = 14nM C2C12 cells.

Endotoxin: 0.1ng/ug (1EU/ug)

Quality Control: Cell culture tested

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Molecular Weight:
Source: Human, Recombinant (E. coli)
Purity: 98% by SDS-PAGE and HPLC analyses.
Form: Supplied as a lyophilized powder. RReconstitute with 50mM sodium acetate, pH 4.3 to 0.1-1mg/ml. This solution can then be diluted into other buffers.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Hilger F., et al., J Biol. Chem. 280: 14974-80 (2005)

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.