Technical Data
pro Bone Morphogenetic Protein 2 (proBMP2), Human, Recombinant
Molecular Biology Storage: -70CShipping: Dry Ice
proBMP-2 is the pro-protein of BMP-2 and processed by proteases into mature BMP-2. ProBMP-2 Human Recombinant is the precursor of BMP-2 which has 3 intramolecular cysteine bridges is a homodimer, non-glycosylated, polypeptide chain containing 337 amino acids and having a molecular mass of 85,726D. Recombinant, Human proBMP-2 administration leads to ectopic bone formation in rats (Hillger et al., 2005). Rh proBMP-2 is recombinantly expressed in Escherichia coli, native protein is obtained upon refolding of inclusion body material.

Function: Induces cartilage and bone formation.

Subunit: Homodimer; disulfide-linked. Interacts with GREM2 (By similarity) and SOSTDC1.

Subcellular Location: Secreted.

Tissue Specificity: Particularly abundant in lung, spleen and colon and in low but significant levels in heart, brain, placenta, liver, skeletal muscle, kidney, pancreas, prostate, ovary and small intestine.

Biological Activity: The ED50 as determined by the stimulating effect on the migration of C3T3 cells and was found active.

Storage and Stability:
Lyophilized PproBMP-2, although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution store at 4C.
Reconstitute lyophilized ProBMP-2 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Molecular Weight:
Source: Recombinant, Human (E. coli).
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Form: Supplied as a lyophilized powder from a concentrated (0.1mg/ml) sterile solution containing 10mM HCL pH2.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Hillger F, Herr G, Rudolph R, Schwarz E., Biophysical comparison of BMP-2, ProBMP-2, and the free
pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. J Biol Chem. 2005; 280:14974-80.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.