Technical Data
B2553-06C
pro Bone Morphogenetic Protein 2 (pro BMP2), Human, Recombinant
10ug
50ug
50ug
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Technical Data |
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B2553-06C |
pro Bone Morphogenetic Protein 2 (pro BMP2), Human, Recombinant |
10ug 50ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: Dry Ice |
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proBMP-2 is the pro-protein of BMP-2 and processed by proteases into mature BMP-2. The role of the pro-sequence that exceeds in length (263 amino acids) the mature part (114 amino acids) is currently not known. The rh proBMP-2 correponds to the authentic natural protein with 377 amino acids. Rh proBMP-2 is recombinantly expressed in Escherichia coli, native protein is obtained upon refolding of inclusion body material. Molecular mass: 85.476kD Biological Activity: Recombinant, Human proBMP-2 administration leads to ectopic bone formation ion rats (Hillger et al., 2005). Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 20mM AcOH with 0.1% HSA or BSA and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 26018D |
Source: Recombinant, Human (E. coli). Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Concentration: ~1mg/ml Form: Supplied as a lyophilized powder in a filtered buffer solution. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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Hillger F, Herr G, Rudolph R, Schwarz E., Biophysical comparison of BMP-2, ProBMP-2, and the free pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. J Biol Chem. 2005; 280:14974-80.
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