Technical Data
pro Bone Morphogenetic Protein 2 (pro BMP2), Human, Recombinant
Molecular Biology Storage: -70CShipping: Dry Ice
proBMP-2 is the pro-protein of BMP-2 and processed by proteases into mature BMP-2. The role of the pro-sequence that exceeds in length (263 amino acids) the mature part (114 amino acids) is currently not known. The rh proBMP-2 correponds to the authentic natural
protein with 377 amino acids.

Rh proBMP-2 is recombinantly expressed in Escherichia coli, native protein is obtained upon refolding of inclusion body material.

Molecular mass: 85.476kD

Biological Activity:
Recombinant, Human proBMP-2 administration leads to ectopic bone formation ion rats (Hillger et al., 2005).

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 20mM AcOH with 0.1% HSA or BSA and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Recombinant, Human (E. coli).
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder in a filtered buffer solution.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Hillger F, Herr G, Rudolph R, Schwarz E., Biophysical comparison of BMP-2, ProBMP-2, and the free
pro-peptide reveals stabilization of the pro-peptide by the mature growth factor. J Biol Chem. 2005; 280:14974-80.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.