Technical Data
B2553-06D
Bone Morphogenetic Protein 2, Recombinant, Human (BMP2)
2ug
10ug
100ug
Molecular Biology Storage: -70CShipping: Blue Ice
BMP-2 belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. Recombinant Human Bone Morphogenetic protein-2 produced in E. coli is a homodimeric, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 26018D.

Endotoxin: 0.01ng/ug or 0.1EU/ug as per LAL.

Biological Activity: BMP-2 is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of MC3T3-E1 cells is less then 50ng/ml.

AA Sequence:
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
2.6kD
Source: Recombinant, human, E. coli
Purity: ~95% Analysis by RP-HPLC, Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.