Technical Data
Bone Morphogenetic Protein 4 (BMP4, DVR4)
Bone Morphogenic Proteins (BMP) are members of the TGF-beta superfamily that affect bone and cartilage formation (1,2,3). Mature BMPs are 30-38kD proteins that assume a TGF-beta -like cysteine knot configuration.Lovostatin increases bone formation by turning on the bmp-2 gene (4). BMPs stimulate the production of specific bone matrix proteins and alter stromal cell and osteoclast proliferation (5,6). BMPs may also be an important factor for development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis (1,7).BMPs appear to be responsible for normal dorsal/ventral patterning. Like TGF-beta, BMPs bind to a type II receptor, which then recruits the transducing type I receptor unit, activating the Smad protein signaling pathway (8,9,10).

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: detects a limit of around 6ng/well of human BMP-4.
Western Blot: Use human BMP-4 at 50ng/lane under non-reducing and reducing conditions.
Neut: 50% of the bioactivity due to 0.5 µg/ml recombinant human BMP-4I
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b8J348Affinity Purified
100ug-20CBlue IceHumanMouse
Recombinant full length protein expressed in NSO cells (Human).
Purified by immunoaffinity chromatography.
No Buffer
Neutralizes human BMP-4, and recognizes recombinant human BMP-4 by ELISA and immunoblotting. The antibody shows approximately 5% crossreactivity with recombinant human BMP-2, recombinant mouse BMP RIA and BMP-RIB. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.