Bone Morphogenetic Protein 4, Recombinant, Human (BMP4, DVR4) (Human 293 Cells)
|Molecular Biology||Storage: -20°CShipping: Blue Ice|
Human Cell Expression Cytokines:
Expression of proteins in our Human 293-cell expression system is a cost-effective and scalable production method for high-authenticity, recombinant human proteins.
Functional activities of human proteins depend on proper folding, phosphorylation, disulfide bridge formation, and proteolytic processing or appropriate glycosylation. Ideally, recombinant human proteins are produced in human cells that are more authentic in terms of both physical properties and biochemical functions. However, the current process of human cell expression requires a large quantity of DNA and medium supplemented with bovine serum. Hence, it is often a daunting task to produce sufficient amount of high quality proteins for drug discovery or diagnostic development (1-100mg) at acceptable cost. United States Biological is now supplying a high yield, cost-effective and versatile system to produce active recombinant human proteins in a human cell line to meet the special needs of the broad scientific community and drug discovery industry. In this system, we have broken key bottleneck steps to increase protein production and reduce cost, including
• Engineered human cell line adapted to suspension for growth in serum-free and chemically defined medium
• Proprietary expression vectors driven by strong promoters and signal peptides
• Large scale transient expression with small quantity of DNA
• Rapid creation of stable human cell lines and adaptation to serum-free medium
• Short time to scale up production
• Efficient tag or tag-free purification
Using this expression system, we have produced a large number of highly active human cytokines, kinases and phosphatases. Our technology is particularly suitable for the production of high-authenticity human proteins in medium scale as research and diagnostic reagents, drug screening targets, and therapeutic proteins for preclinical evaluation. Key differences of recombinant human protein production in different systems are illustrated below:
Expression system E. coli Insect cell CHO cell Human cell
Protein folding + ++ +++ ++++
Phosphorylation - ++ +++ ++++
Proteolytic processing - + +++ ++++
Glycosylation - Poor Not human-like Authentic
Bone Morphogenetic Protein 4 (BMP-4) belongs to the bone morphogenetic protein family that is a subgroup of the TGF-beta superfamily. BMP-4 is a 34kD glycosylated homodimer with a disulfide linkage. BMP-4 provides ventralizing signals for existing mesoderm and has a crucial role in mammalian hematopoietic stem cells. This cytokine is important in the onset of endochondral bone formation in humans and is expressed from early embryogenesis through adulthood. BMP-4 is essential in epidermal determination, the develpment of mutiple organs, tissue repair, mesenchyme formation, and suppression of neural induction.
The specific activity was determined by its ability to induce alkaline phosphatase
production in a dose response to BMP-4 in the ATDC-5 cell line (Mouse chondrogenic cell line).
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 4mM HCl, 0.1% recombinant HSA (A1327-54A). Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: HEK 293 cells
Purity: 95% as determined by SDS-PAGE and HPLC. Endotoxin: 1EU/ug (LAL).
Form: Supplied as a lyophilized powder in PBS. Reconstitute with 4mM HCl, 0.1% endotoxin-free recombinant HSA.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.