Technical Data
Bone Morphogenetic Protein 4 (BMP 4, BMP4, BMP-4, BMP 2B, BMP2B, BMP-2B, BMP2B1, DVR4, ZYME)
Bone morphogenetic protein 4 (BMP4) is one of nine structurally related BMPs within the transforming growth factor beta superfamily. BMP4 stimulates bone formation in adult mammals and also functions as a signaling molecule in embryonic nervous system, musculature and skeleton. It also seems involved with early tooth morphogenesis, and is expressed in prostate adenocarcinoma and benign prostatic hyperplasia. This antibody will help detect BMP4 in a variety of tissues.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:25-1:50
Immunohistochemistry: 1:10-1:20 for 1 hour at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Saos-2 cell lysate
Immunohistochemistry: Prostatic adenocarcinoma and osteoclastoma, cytoplasmic staining pattern.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
1ml-20CBlue IceMouseMouse
Not determined
Recombinant mouse bone morphogenetic protein 4.
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml of dH2O.
Recognizes Bone Morphogenetic Protein 4. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Anderson H C, Hsu H H T, Raval P, et al.. The mechanism of bone induction and bone healing by human osteosarcoma cell extracts. Clinical Orthopaedics and Related Research. 313:129-134 (1995). 2.Heikinheimo K. Stage-specific expression of decapentaplegic-Vg-related Genes 2, 4, and 6 (bone morphogenetic proteins 2, 4, and 6) during human tooth morphogenesis. J. Dent. Res. 73(3):590-597 (1994). 3. Bentley H, Hamdy F C, Hart K A, et al.. Expression of bone morphogenetic proteins in human prostatic adenocarcinoma and benign prostatic hyperplasia. Br. J. Cancer. 66:1159-1163 (1992). 4.Jones C M, Lyons K M, Lapan P M , et al.. DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction. Development. 115:639-647 (1992). 5.Jones C M, Lyons K M and Hogan B L M. Involvement of bone morphogenetic protein-4 (BMP-4) and Vgr-1 in morphogenesis and neurogenesis in the mouse. Development. 111:531-542 (1991).