		Technical Data
B2553-21A	Bone Morphogenetic Protein 4, 293-408aa, Recombinant, Human (BMP4, DVR4)	100ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Bone morphogenetic protein 4 (BMP-4) is a member of the bone morphogenetic protein family which belongs to the TGF- superfamily. This protein is a vital regulatory molecule that functions throughout bone and cartilage development, specifically tooth development, limb formation, bone induction, and fracture repair. In human embryonic development, BMP- 4 is required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. And it is overexpressed in patients with fibrodysplasia ossificans progressiva. Recombinant BMP-4 was expressed as insoluble protein aggregate in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Endotoxin Level:
1.0 EU per 1 ug of protein (determined by LAL method)15% SDS-PAGE (3ug)

Molecular Weight: 13.2kD (117 aa)

AA Sequences:
MSPKHHSQRA RKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI
SMLYLDEYDK VVLKNYQEMV VEGCGCR

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
13.2kD

Source: E. coli
Purity: 95% by SDS-PAGE
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 10mM sodium citrate buffer, pH 3.5, 10% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Moon BS., et al. (2009) Exp Mol Med. 41(2):116-25. 2. Li BC., et al. (2009) J Trauma. 66(2):450-6

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.