Technical Data
B2553-22
Bone Morphogenetic Protein 4 (BMP4, DVR4)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: To detect hBMP-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 4µg/ml of this antibody is required. This monoclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.1ng/well of recombinant hBMP-4.
Western Blot: To detect hBMP-4 by Western Blot analysis this antibody can be used at a concentration of 0.25ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-4 is 3.91ng/lane, under non-reducing conditions.
Optimal dilutions to be determined by the researcher.

Reconstitution:
Reconstitute to 1mg/ml with sterile dH2O or sterile 40-50% glycerol for long term storage.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA
TypeIsotypeCloneGrade
MabIgG2b7F2Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified recombinant, E. coli derived, human BMP-4
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS.
Specificity:
Recognizes human Bone Morphogenic Protein 4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.