Technical Data
B2553-32A
Bone Morphogenetic Protein 7, Recombinant, Human, Western Blot Positive Control (BMP7)
100ul
Molecular Biology Storage: -20CShipping: Blue Ice
Bone Morphogenetic Proteins belong to the TGF-beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type I and II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1/GDF-5), other members, such as BMP-3, -6, -7 and 13 (CDMP-2/GDF-6) may be involved in later stages of skeletal formation.

BMP7 or OP1, a 431-amino acid polypeptide that includes a secretory signal sequence, (Chr 20), is expressed in kidney, bladder and brain. Induces cartilage and bone formation may be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. It plays a role in calcium regulation and bone homeostasis.

Source:
Recombinant Human BMP-7P-2 signal peptide and propeptides (aa1-282 of human BMP-2) fused to the human BMP-7 mature chain (aa293-431 of human BMP-7) was expressed in Chinese hamster ovary cell line. The mature recombinant human BMP-7, generated by the proteolytic removal of the signal peptide and propeptide, is a disulfide-linked homodimeric protein consisting of two 139aa residue subunits with a MW of ~15.7kD. Due to glycosylation the rhBMP-5 migrates as an ~30-40kD protein under non-reducing conditions and as 18-20kD protein under reducing conditions in SDS-PAGE.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: Load 10ul/lane of human BMP-6 for good visibility with antibodies.
Optimal dilutions to be determined by the researcher.

Note: SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity: Purified
Concentration: As reported
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.) Sampath, T. K et al (1990) JBC 265: 13198-13205,
2.) Reddi, A.H et al (1998), Nature Biotechnology 16: 247-252;
3.) Helder, M. N et al (1995) J. Histochem. Cytochem 43: 1035-1044;
4.) Godin, R. E et al (1998) Development 125: 3473-3482.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.