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Technical Data |
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B2553-33H |
Bone Morphogenetic Protein 7 (BMP7, OP-1, Osteogenic Protein-1) (Biotin) |
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Description: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Direct, Sandwich ELISA: 0.25-1ug/ml (using 100ul/well antibody solution).Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4ng/well of recombinant hBMP-7/OP-1. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBMP-7/OP-1 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, PBS, 0.1% BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA |
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