Technical Data
B2553-37AX
Bone Morphogenetic Protein 10, Recombinant, Human (BMP10) (BSA Free)
25ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
The mature recombinant human BMP-10, generated by the proteolytic removal of the signal peptide and the propeptide, is a disulfide-linked homodimeric protein. Based on N-terminal sequencing, the mature recombinant human BMP-10 starts at Asn 317. The monomer has a calculated molecular mass of 12.2kD and migrates as an approximately 12.2kD protein in SDS-PAGE under reducing conditions.

Source:
A DNA sequence encoding human BMP-10 (Met 1-Arg 424; Accession # O95393) was expressed in a Chinese hamster ovary (CHO) cell line.

Endotoxin:
< 1.0 EU per 1 g of the protein as determined by the LAL method.

Activity:
Measured by its ability to induce alkaline phosphatase synthesis by MC3T3E1 osteoblastic cells (Mazerbourg, S. et al., 2005, J. Biol. Chem. 280:32122). The ED50 for this effect is typically 15-60ng/mL.

Reconstitution:
It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100ug/ml. The carrier-free protein should be used immediately upon reconstitution to avoid losses in activity due to non-specific binding to the inside surface of the vial. For long term storage as a dilute solution, a carrier protein (e.g. 0.1% HSA or BSA) should be added to the vial.

Storage:
Lyophilized samples are stable for up to twelve months at -20C. Upon reconstitution, this protein can be stored under sterile conditions at 2-8C for one month or at -20C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Source: Chinese hamster ovary (CHO) cells
Purity: 97%, as determined by SDS-PAGE and visualized by silver stain.
Concentration: As reported
Form: Supplied as a lyophilized powder in 30% acetonitrile, 0.1% TFA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.