Technical Data
B2553-50E
Bone Morphogenetic Protein 3, Recombinant, Human, Western Blot Positive Control (BMP3)
100ul
Molecular Biology Storage: -20CShipping: Blue Ice
The BMPs belong to the TGF- Beta superfamily, whose members are widely represented throughout the animal kingdom. The BMPs are important regulators of key events in the processes of bone formation during embryogenesis, postnatal growth, remodeling and regeneration of the skeleton. The BMPs function by binding to a receptor complex that is found on all normal cells and is composed of type-I and II receptors. The primary unit of bone formation is osteoblast, the bone-forming cell. These osteoblast cells respond to physical loading by transducing signals that alter gene expression patterns, and Cbfa (core binding factor), the osteoblast specific transcription factor plays an important role in osteoblast differentiation and function.
Localization studies in both human and mouse tissues have demonstrated high levels of mRNA expression and protein synthesis for various BMPs in kidney, heart, lung, small intestine, limb bud and teeth. Several BMPs have been implicated in early skeletal development, including BMP-2, -4, -5, -7, -14 (CDMP-1 / GDF-5), other members, such as BMP-3, -6, -7 and 13 (CDMP-2 / GDF-6) may be involved in later stages of skeletal formation.
BMP3 / Osteogenin/ BMP-3A is a 472aa protein in human (chr 4p14-q21), highly expressed in lung, ovary and small intestine, Its function is involved in the cartilage and bone formation. BMP3 and BMP2 genes map to conserved regions between human and mouse.
BMP-3B / GDF-10, a 478aa protein in human (Chr 10q11.22), belongs to a group of proteins that can induce endochondral bone formation in adult animals, it is closely related in sequence to BMP3 with 44% homology. The amino acid sequences of human and rat BMP-3b precursor proteins are 83% similar, whereas the mature proteins show 98% identity. BMP-3B is mainly expressed in femur, brain, lung, pancreas and testis.

Source:
Recombinant human BMP3, expressed in E.coli. The active human BMP-3 refolded from monomer is a disulfide-linked homodimer. Each methionyl form of the monomer consists of 111aa and has a predicted molecular mass of 12.5kD.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: Load 10ul/lane for good visibility with antibodies.
Optimal dilutions to be determined by the researcher.

Note: SDS may crystallize in cold conditions. It should redissolve by warming before taking it from the stock. It should be heated once prior to loading on gels.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
12.5kD
Purity: 95%
Concentration: As reported
Form: Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1.) Kawabata, M et al (1998) Cytokine and Growth Factor Reviews 9: 49-61,
2.) Ebendal, T et al (1998), J. Neurosci. Res. 51: 139-146;
3.) Reddi, A. H (1998), Nature Biotechnology 16: 247-252;
4.) Daluiski, A et al (2001), Nature Genetics 27: 84;
5.) Bahamonde, M. E et al (2001) Bone and Joint Surgery 83-A (suppl 1):

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.