Technical Data
B2553-60
Bone Morphogenic Protein Receptor 1A (CD292, BMPR-1A, Bone Morphogenetic Protein Receptor Type IA, Serine Threonine Protein Kinase Receptor R5, SKR5, Activin Receptor-like Kinase 3, ALK-3, ACVRLK3)
Description:
Bone Morphogenetic Proteins (BMPs) are members of the TGF- superfamily of cytokines that affect bone and cartilage formation1. Mature BMPs are 30-38kD proteins that assume a TGF beta-like cysteine knot configuration1. Most BMPs are homodimers, but bioactive natural heterodimers have also been reported1. BMPs are involved in embryogenesis and
morphogenesis of various tissues and organs. They create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. BMPs also regulate the growth, differentiation, chemotaxis, proliferation, and apoptosis of mesenchymal cells, epithelial cells,
hematopoietic cells, and neuronal cells, and are responsible for normal dorsal/ventral patterning. Two type I receptors have been characterized, BMPR-IA (also designated activin receptor-like kinase ALK-3, BRK-1 and SKR5), and BMPR-IB (also designated ALK-6 and SKR 6), that bind to bone morphogenetic proteins (BMP)-2, BMP-4, and osteogenic protein (OP)-1 (also designated BMP-7)2-5. Type I receptors involved in BMP signaling can independently bind the
various BMP family proteins in the absence of type II receptors. BMPR-IA and BMPR-IB are thought to mediate distinct effects on gene expression, cell differentiation, and morphogenesis in a dose dependent fashion 2,6.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunoprecipitation: 7ug/reaction
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~0.25 mg/ml
Immunogen:
Synthetic peptide derived from an internal region of the human BMPR-1A (bone morphogenetic protein receptor type 1A, Serine/threonine protein kinase receptor R5 (SKR5), activin receptor-like kinase-3 (ALK-3)), which differs from mouse and rat BMPR-1A by o
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human BMPR-1A at ~60 kD. Reactivity has been confirmed with human SK-OV-3, NGP96, SKMEL-19, SK-MEL-37, A375, MeWo cell lysates and mouse ovary homogenates. Based on amino acid sequence homology, reactivity with rat is also expected. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hogan BL, Genes Dev 10:1580-1594, 1996.
2. Rosenzweig BL, et al. PNAS 92:7632-7636, 1995.
3. Ten Dijke P, et al. J Biol Chem 269:16985-16988, 1994.
4. Yamada N, et al. Br J Cancer 73: 624-629, 1996.
5. Yonemori K, et al. Am J Pathol 150:1335-1347, 1997.
6. Soderstrom S, et al. Cell Tissue Res 286:269-279, 1996.
7. Reddi AH. Nat Biotechnol 16:247-252, 1998.
8. Howe JR, et al. Nat Genet 28:184-187, 2001.
9. Zhou XP, et al. Am J Hum Genet 69:704-711, 2001.
10. Eng C. Nat Genet 28:105-107, 2001.