		Technical Data
B2553-60B	Bone Morphogenic Protein Receptor 1A (CD292, BMPR-1A, Bone Morphogenetic Protein Receptor Type IA, Serine Threonine Protein Kinase Receptor R5, SKR5, Activin Receptor-like Kinase 3, ALK-3, ACVRLK3)

Description:
The bone morphogenetic protein (BMP) receptors are a family of transmembrane serine/threonine kinases that include the type I receptors BMPR1A and BMPR1B and the type II receptor BMPR2. These receptors are also closely related to the activin receptors, ACVR1 and ACVR2. The ligands of these receptors are members of the TGF-beta superfamily. TGF-betas and activins transduce their signals through the formation of heteromeric complexes with 2 different types of serine
(threonine) kinase receptors: type I receptors of about 50-55kD and type II receptors of about 70-80kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:100-1:500 to detect BMPR1A in Hela cell lysate.
Immunohistochemistry: 1:50-1:100. Formalin-fixed and paraffin-embedded human cancer tissue was stained with B2553-60B followed by peroxidase-conjugate secondary antibody and DAB staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic peptide cooresponding to aa 173-202 from the central region of human BMPR1A, conjugated to KLH. MW: 60198D

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Specificity:

Recognizes human Bone Morphogenic Protein Receptor 1A (BMPR-1A). Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Zhou, X.-P., et al., Am. J. Hum. Genet. 69(4): 704-711 (2001). 2. Howe, J.R., et al., Nat. Genet. 28(2): 184-187 (2001). 3. ten Dijke, P., et al., Oncogene 8(10): 2879-2887 (1993).