Technical Data
B2554-15
BORG2 (Binder of Rho GTPases 2, CDC42 Effector Protein (Rho GTPase Binding) 3, Cdc42 Effector Protein 3, CDC42EP3, CEP3, ORG2, UB1)
Description:
CDC42, a small Rho GTPase, regulates the formation of F-actin-containing structures through its interaction with the downstream effector proteins. The protein encoded by this gene is a member of the Borg family of CDC42 effector proteins. Borg family proteins contain a CRIB (Cdc42/Rac interactive-binding) domain. They bind to, and negatively regulate the function of, CDC42. This protein can interact with CDC42, as well as with the ras homolog gene family, member Q (ARHQ/TC10). Expression of this protein in fibroblasts has been shown to induce pseudopodia formation.

Applications:
Suitable for use in Immunoprecipitation. Not recommended for Western Blot. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2-5ug/mg lysate
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C Do Not FreezeBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
The epitope recognized by this antibody maps to a region between residue 125 and 175 of human binder of Rho GTPases 2 using the numbering given in entry NP_006440.2
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris-citrate/phosphate, pH 7.5, 0.09% sodium azide.
Specificity:
Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.