Technical Data
B2570-12
Borrelia burgdorferi (Lyme Disease)
Description:
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.

B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.

Applications:
Suitable for use in ELISA, Fluorescence Microscopy, Western Blot, Immunohistochemistry and Immunocytochemistry. It is also suitable for Conjugation purposes. Other applications not tested.

Recommended Dilutions:
Western Blot: >1:1,000
Avidin-biotin IFA: >1:40,000
Immunohistochemistry: Formalin-fixed paraffin-embedded sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1ml-20CBlue IceRabbit
Concentration:
~4-5mg/ml
Immunogen:
Whole cell preparation from B. burgdorferi.
Purity:
Purified IgG fraction to a purity of ~95%.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Specificity:
Recognizes Borrelia burgdorferi at 83kD, 41kD, 34kD and 31kD, and additional low MW bands. Crossreactivity: Treponema pallidum, B. hermsii & B. parkerii.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.