Technical Data
Borrelia burgdorferi (Lyme Disease)
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response.

B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.

Suitable for use in ELISA, Fluorescence Microscopy, Western Blot, Immunohistochemistry and Immunocytochemistry. It is also suitable for Conjugation purposes. Other applications not tested.

Recommended Dilutions:
Western Blot: >1:1,000
Avidin-biotin IFA: >1:40,000
Immunohistochemistry: Formalin-fixed paraffin-embedded sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGHighly Purified
1ml-20CBlue IceRabbit
Whole cell preparation from B. burgdorferi.
Purified IgG fraction to a purity of ~95%.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins have been added.
Recognizes Borrelia burgdorferi at 83kD, 41kD, 34kD and 31kD, and additional low MW bands. Crossreactivity: Treponema pallidum, B. hermsii & B. parkerii.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.