Technical Data
B2570-15
Borrelia burgdorferi (Lyme Disease)
Description:
Borrelia burgdorferi is a spirochete and the cause of Lyme disease, a tick transmitted illness of humans and animals. B. burgdorferi may persist in humans and animals for months or years following initial infection, despite a robust humoral immune response. B. burgdorferi resembles other spirochetes in that it is a highly specialized, motile, two-membrane, spiral shaped bacteria which lives primarily as an extracellular pathogen. B. burgdorferi has an unusual genome compared with other eubacteria which includes a linear chromosome approximately one megabase in size and numerous linear and circular plasmids.

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Also suitable for conjugation purposes. Other applications have not been tested.

Recommended Dilutions:
Immunofluorescence: Acetone-fixed
Immunohistochemistry: Formalin-fixed/paraffin-embedded sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1ml-20°CBlue IceRabbit
Concentration:
4-5mg/ml
Immunogen:
Whole cell preparation from B. burgdorferi. Strain B31 ATCC #35210.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added.
Specificity:
Recognizes Borrelia burgdorferi at 83kD, 41kD, 34kD and 31kD and additional low MW bands. Species Crossreactivity: Treponema pallidum, B. hermsii and B. parkerii.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Marques A. R. et al., Clinical and Diagnostic Laboratoy Immunology 12(9):1036-1040 (2005)