Technical Data
B2700
Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
2ug
10ug
Growth Factors, Cytokines Storage: -20CShipping: RT
BDNF is a member of the NGF family of neurotrophic growth factors. Like other members of this family, BDNF supports neuron proliferation and survival. BDNF can bind to a low affinity cell surface receptor called LNGFR, which also binds other neurotrophins such as NGF, NT-3 and NT-4. However, BDNF mediates its neurotrophic properties by signaling through a high affinity cell surface receptor called gp145/trkB. BDNF is expressed as the C-terminal portion of a 247 amino acid polypeptide precursor, which also contains a signal sequence of 18 amino acid residues and a propeptide of 110 amino acid residues.

Recombinant human BDNF is a 27.0kD homodimer of two 119 amino acid subunits linked by strong non-covalent interactions. Human and mouse BDNF sequences are identical.

Biological Activity:
Biological activity was determined by its ability to stimulate the proliferation of rat C6 cells. The expected ED50 for this effect is 0.5-1ug/ml.

AA Sequence:
MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Country of Origin:
USA

Molecular Weight:
27.0kD
Source: Human, recombinant protein expressed in E. coli
Purity: 98% (SDS-PAGE, HPLC) Endotoxin: 0.1ng/ug (1EU/ug)
Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
US Biological application reference: Connolly, A.M. et al., (2006) Biological Psychiatry 59:354-363 (E).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.