Technical Data
Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF)
Molecular Biology Storage: -20CShipping: RT
Human Brain-Derived Neurotrophic Factor is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids. The sequence of the first five N-terminal amino acids was determined to be Met-His-Ser-Asp-Pro. Contains less than 1% dimers and aggregates.

Recombinant Human Brain-Derived Neurotrophic Factor expressed in E. coli.

Molecular Weight: 26,984

The ED50 as determined by the dose dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50ng/ml.

Endotoxin: 0.1 ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with ddH2O. Aliquot and store at -20C. For long-term storage, add a carrier protein (0.1% HSA or BSA), Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~96% (HPLC, FPLC, reducing and non-reducing SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from 20mM sodium citrate buffer, pH 5.0. No preservative added. Reconstitute using sterile deionized water to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.