Technical Data
B2700-01K
Brain-Derived Neurotrophic Factor, Recombinant, Human (BDNF, Abrineurin, MGC34632)
2ug
Molecular Biology Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 2x119aa of human Brain-derived Neurotrophic Factor expressed in E.coli is a homodimer, non-glycosylated, polypeptide chain.

Molecular Weight:
~27kD

Biological Activity:
The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase)in rat basal forebrain primary septal culture is 50nug/ml

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BDNF as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~27kD
Purity: ~96% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: Not determined
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. NMDA receptor activation modulates programmed cell death during early post-natal retinal development: a BDNF-dependent mechanism. J Neurochem 2005 Oct;95(1):244-53. 2. Cellular levels of TrkB and MAPK in the neuroprotective role of BDNF for embryonic rat cortical neurons against hypoxia in vitro. Int J Dev Neurosci 2005 Oct;23(6):515-21. 3. Increased expression of BDNF and proliferation of dentate granule cells after bacterial meningitis. J Neuropathol Exp Neurol 2005 Sep;64(9):806-15. 4. BDNF acutely modulates synaptic transmission and calcium signalling in developing cortical neurons. Cell Physiol Biochem 2005;16(1-3):69-76. 5. Area-specific effects of brain-derived neurotrophic factor (BDNF) genetic ablation on various neuro nal subtypes of the mouse brain. Brain Res Dev Brain Res 2005 May 12;156(2):111-26. 6. The change of the neuron-glia differentiation rate in human neural precursor cells (HPCs) and Ad-BDNF-/-GDNF-infected HPCs following the administration of a neurotoxin. Neurosci Lett 2005 Oct 21;387(2):100-4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.