Technical Data
Media
Brain Heart Infusion Agar (Powder)
B2701-05
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Brain Heart Infusion (from solids)8.0
Peptic Digest of Animal Tissue5.0
Pancreatic Digest of Casein16.0
Sodium Chloride5.0
Dextrose 2.0
Disodium Phosphate2.5
Agar13.5
Total:52.0g/liter
Brain Heart Infusion Agar is used for cultivating a wide variety of fastidious and non-fastidious microorganisms, including bacteria, yeasts and fungi

Description:
Brain Heart Infusion (BHI) Agar has proved to be effective in the cultivation of a wide variety of microorganisms, including many types of pathogens. BHI Agar is currently recommended as a general medium for aerobic bacteria and for the primary recovery of fungi from clinical specimens. BHI Agar, with 10% Sheep Blood can be used to isolate systemic fungi that may grow poorly on nonenriched media. A high recovery rate for Histoplasma capsulatum can be obtained with the addition of horse blood to the medium.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions per Liter: Dissolve 52 grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability::Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Beige homogeneous, free flowing powder pH (5.2%, 25C)

Microbiological Analysis: Aspergillus niger ATCC 16404


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.