Technical Data
Brain-Heart Infusion Broth w/o Dextrose, NaCl (Powder)
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation/Liter: Shown as g/L
Brain Heart Infusion17.5
Enzymatic Digest of Gelatin10
Sodium ChlorideAbsent
Disodium Phosphate2.5
Total: 30g/L
Brain-Heart Infusion Broth is used for the cultivation of a wide variety of fastidious organisms.
Rosenow1 prepared a rich medium for culturing streptococci by combining dextrose broth and brain tissue. Hayden2 modified the original formula while working with dental pathogens. The current formula is a modification of Rosenow1 and Hayden2, using dehydrated infusions of porcine brain and heart tissue. Brain-Heart Infusion Broth can be supplemented with antibiotics, varying amounts of sodium chloride, yeast extract, and serum to provide a rich medium for bacteria, yeasts and pathogenic fungi.3 The addition of 0.1% agar can be used to lower oxygen tension, providing an atmosphere to support the growth of aerobic, microaerophilic, and obligate anaerobic microorganisms. Brain-Heart Infusion Broth, abbreviated as BHI, is specified in many references for food and water testing.4-7 NCCLS, National Committee for Clinical Laboratory Standards, cites Brain-Heart Infusion Broth for preparing the inoculum used in antimicrobial susceptibility tests.8

The nitrogen, vitamin, and carbon sources are provided by Brain-Heart Infusion and enzymatic digest of gelatin in BHI Broth. Dextrose is the carbohydrate source, and sodium chloride maintains the osmotic environment. Disodium phosphate is the buffering agent in this medium.

Both dextrose and sodium chloride have been removed in order to allow the researcher to control the experimental conditions.

Yellow to beige, free flowing, homogenous powder

Aamber to clear, complete with heating.


Growth Supporting Properties:
Peptone Agar (2%):
E. coli ATCC 25922: good to excellent recovery
S. aureus ATCC 25923: fair to good recovery

Country of Origin:
Brain: Porcine, USA
Heart: Porcine, USA

Directions per Liter: Suspend 37g of the medium in one liter of distilled or deionized water. Heat with frequent agitation and boil for one minute to completely dissolve the medium. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.Note: Both dextrose and sodium chloride have been removed in order to allow the researcher to control the experimental conditions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Rosenow, E. C. 1919. Studies on elective localization. J. Dent. Research 1:205-249. 2. Hayden, R. L. 1923. Elective localization in the eye of bacteria from infected teeth. Arch. Int. Med. 32:828-849. 3. Atlas, R. M. 1993. Handbook of microbiological media, p. 147-153, CRC Press, Boca Raton, FL. 4. Cunnif, P. (ed.). 1995. Official Methods of Analysis AOAC International, 16th ed. AOAC International, Gaithersburg, MD. 5. U.S. Food and Drug Administration. Bacteriological analytical manual, 8th ed., AOAC International, Gaithersburg, MD. 6. Vanderzant, C., and D. F. Splittstoesser (eds.). 1992. Compendium of methods for the microbiological examination of food., 3rd ed. American Public Health Association, Washington, D.C. 7. Greenberg, A. E., L. S. Clesceri, and A.D. Eaton (eds.). 1995. Standard methods for the examination of water and wastewater,
19th ed. American Public Health Association, Washington, D.C. 8. National Committee for Clinical Laboratory Standards. 1994. M11-A3, Vol. 13, No. 26, Methods for antimicrobial susceptibility testing of anaerobic bacteria. National Committee for Clinical Laboratory Standards, Villanova, PA.