Technical Data
Brain Natriuretic Peptide, Pro, NT, aa1-12 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)
In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134aa precursor (prepro-BNP), which is cleaved by proteases to form a 26aa "signal" peptide and a 108aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76aa amino-terminal NT-proBNP and biologically active 32aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Capture: B2702-04T Brain Natriuretic Peptide, Pro, NT, 61-76
Detection: B2702-01B Brain Natriuretic Peptide, Pro, NT, aa1-12

Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG2a9L424Affinity Purified
1mg-20CBlue IceHumanMouse
Synthetic peptide corresponding to aa1-12 of human proBNP.
Purified by Protein A affinity chromatography. >90% pure (SDS-PAGE)
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes human proBNP and NT-proBNP (recombinant and synthetic).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.