Technical Data
B2702-01C
Brain Natriuretic Peptide, Pro, NT, aa13-27 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)
Description:
In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134aa precursor (prepro-BNP), which is cleaved by proteases to form a 26aa "signal" peptide and a 108aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76aa amino-terminal NT-proBNP and biologically active 32aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG19L425Affinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~7mg/ml
Immunogen:
Synthetic peptide corresponding to aa13-27 of the proBNP sequence.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human proBNP and NT-proBNP (recombinant and synthetic) (aa13-27).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.