Technical Data
B2702-03L
Brain Natriuretic Peptide, Pro, NT, 1-12 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)
Description:
In cardiac tissue brain natriuretic peptide (BNP) is synthesized as 134 amino acid precursor (prepro-BNP), which is cleaved by proteases to form a 26 aa "signal" peptide and a 108 aa pro-BNP. Proteolytic digestion of pro-BNP results in formation of 76 aa amino-terminal NT-proBNP and biologically active 32 aa BNP hormone molecule. Both proBNP and NTproBNP circulate in human plasma and have been proposed as markers for early diagnosis of left ventricular dysfunction as well as prognostic markers of possible cardiac complications at patients with heart failure.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:2000
Western Blot: 1:100-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG18J362Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
Immunogen:
Synthetic peptide corresponding to N terminal aa1-12, HPLGSPGSASDL, of Human proBNP. Cellular Localization: Secreted
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Brain Natriuretic Peptide, Pro at ~13kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Pfister R et al. Use of NT-proBNP in routine testing and comparison to BNP. Eur J Heart Fail 6:289-93 (2004). Hall C Essential biochemistry and physiology of (NT-pro)BNP. Eur J Heart Fail 6:257-60 (2004). Hobbs FD et al. Reliability of N-terminal pro-brain natriuretic peptide assay in diagnosis of heart failure: cohort study in representative and high risk community populations. BMJ 324:1498 (2002).