Technical Data
B2702-03V
Brain Natriuretic Peptide, Pro, NT, 13-27 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)
Description:
Human brain natriuretic peptide (BNP) is a secreted protein which is a member of the natriuretic peptide family. BNP is a cardiac hormone, which is synthesized as a pro-hormone (proBNP), and is proteolytically cleaved to release a biologically active fragment (BNP), and an inactive fragment (NT-proBNP) into the circulation.

BNP is predominantly secreted from the cardiac ventricles in response to volume and pressure overload, and results in a number of biological activities including natriuresis, diuresis, vasorelaxation, and inhibition of the sympathetic nervous system. A high concentration of BNP in the bloodstream is indicative of heart failure.

Cellular Localization: Secreted

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a8J368Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
Immunogen:
Synthetic peptide: ETSGLQEQRNHLQGK, corresponding to N terminal amino acids 13-27 of Human proBNP.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Species Crossreactivity: Crossreacts with Human.Not yet tested in other species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Pfister R et al. Use of NT-proBNP in routine testing and comparison to BNP. Eur J Heart Fail 6:289-93 (2004). Hall C Essential biochemistry and physiology of (NT-pro)BNP. Eur J Heart Fail 6:257-60 (2004). Hobbs FD et al. Reliability of N-terminal pro-brain natriuretic peptide assay in diagnosis of heart failure: cohort study in representative and high risk community populations. BMJ 324:1498 (2002).