Technical Data
Brain Natriuretic Peptide, Pro, NT, aa13-27 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)
NT-proBNP and proBNP immunoassay. Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Matched Pair:
Coating Conjugate:
B2702-04F B2702-04P, B2702-04Q B2702-04P, B2702-04Q B2702-04R; The pairs recognize the antigen in blood of patients with unstable angina and myocardial infarction.

Mabs, specific to other epitopes, could also be used in sandwich immunoassay. Possible Mab combinations tested with antigen from patient's blood: B2702-04Q B2702-04S, B2702-04D B2702-04P, B2702-04K B2702-04P, B2702-04T B2702-04H, B2702-04T B2702-04S

Clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Synthetic NT-proBNP peptide, corresponding to aa13-27.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b5E520Affinity Purified
1mg-20CBlue IceHumanMouse
Synthetic NT-proBNP peptide, corresponding to aa13-27. The peptide used for immunization was conjugated with carrier protein.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Human proBNP and NT-proBNP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.