Technical Data
B2702-04Q
Brain Natriuretic Peptide, Pro, NT, aa13-27 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)
Description:
Applications:
Suitable for use in NT-proBNP and proBNP immunoassay . Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice.

Recommended Pair:
Coating-Conjugate:
B2702-04F-B2702-04P
B2702-04Q-B2702-04P
B2702-04Q-B2702-04R
The pairs recognize the antigen in blood of patients with unstable angina and myocardial infarction.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Mabs, specific to other epitopes, could also be used in sandwich immunoassay. Possible Mab combinations tested with antigen from patient's blood: B2702-04Q – B2702-04S, B2702-04D – B2702-04P, B2702-04K – B2702-04P, B2702-04T – B2702-04H, B2702-04T – B2702-04S
TypeIsotypeCloneGrade
MabIgG15E529Affinity Purified
SizeStorageShippingSourceHost
500ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
~3mg/ml
Immunogen:
Synthetic NT-proBNP peptide, corresponding to aa13-27. The peptide used for immunization was conjugated with carrier protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human proBNP and NT-proBNP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.