		Technical Data
B2702-04T	Brain Natriuretic Peptide, Pro, NT, 61-76 (NT-proBNP, N-terminal prohormone brain natriuretic peptide)

Description:
Applications:
Suitable for use in Western Blot and ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Recommended Pair:
Suitable for use as the capture or solid phase antibody.
Matched to B2702-01K as the detection or conjugate (label) antibody.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice immunized with Synthetic NT-proBNP peptide, corresponding to aa61-76.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	5E865	Affinity Purified

Size		Storage	Shipping	Source	Host
500ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~4.5mg/ml

Immunogen:

Synthetic NT-proBNP peptide, corresponding to aa61-76. The peptide used for immunization was conjugated with carrier protein.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.

Specificity:

Recognizes human proBNP and NT-proBNP.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.