Technical Data
Brain Natriuretic Peptide, Pro, NT (BNP)
Brain-type Natriuretic Peptide (BNP) is a nonglycosylated peptide that is produced predominantly by ventricular myocytes and belongs to the natriuretic peptide family. Proteolytic cleavage of the 12kD BNP precursor gives rise to N-terminal proBNP (NT-proBNP) and mature BNP. Plasma NT-proBNP is a marker for congestive heart failure, while mature BNP (aa103-134) promotes vasodilation and fluid and sodium excretion.

Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1ug/ml. The detection limit is ~20ng/well for recombinant human BNP.
Immunohistochemistry: 25ug/ml detects proBNP in paraffin-embedded normal human heart tissue sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG2b8H160Affinity Purified
100ug-20CBlue IceHumanRat
E. coli-derived recombinant human proBNP (aa27-134, Accession #P16860). Species Sequence Homology: porcine (51%), mouse (29%).
Purified by Protein G affinity chromatography.
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Recognizes human proBNP. Does not react with BNP-32 (aa103-134).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.