Technical Data
B2702-29F
Brain Natriuretic Peptide (BNP)
Description:
Brain natriuretic peptide (BNP) circulates in blood as a peptide hormone with natriuretic, vasodilatory and renin inhibitory properties. BNP is secreted predominantly by the left ventricular myocytes in response to volume expansion and pressure overload. BNP belongs to a family of structurally similar peptide hormones, which includes atrial natriuretic peptide (ANP), BNP, C-type natriuretic peptide (CNP) and urodilatin. These peptides are characterized by a common 17aa ring structure with a disulfide bond between two cystein residues. This ring structure shows high homology between different natriuretic peptides (11 of the 17aa residues are homologous in the ring of each of the natriuretic peptides). BNP is a 32aa peptide with disulfide bond between the cysteine residues Cys10 and Cys26. In earlier studies it has been demonstrated that BNP concentration in blood increases with the severity of congestive heart failure. Quantitative measurement of BNP in blood provides an objective indicator of congestive heart failure severity.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells of Balb/c mice

Recommended Pair:
Capture: B2702-29G, B2702-29F
Detection: B2702-29F, B2702-29L

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15E513Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Synthetic BNP peptide corresponding to aa11-22 FGRKMDRISSSS, conjugated with carrier protein.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human BNP and proBNP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.