		Technical Data
B2709-06B	BRCA1 (Breast Cancer Type 1 Susceptibility Protein, RING Finger Protein 53, RNF53)

Description:
The BRCA1 tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is one of the first proteins recruited to sites of DNA double-strand breaks, and serves as part of a scaffold for assembling other DNA damage response or repair factors.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 5-15ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Sheep

Concentration:

~0.1mg/ml after reconstitution

Immunogen:

Recombinant corresponding to aa1634-1863 from C-terminus of human BRCA1 expressed in E.coli.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.

Specificity:

Recognizes human BRCA1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.