Technical Data
B2727-01A
BRCC36, NT (Lys-63-specific Deubiquitinase BRCC36, BRCA1-A Complex Subunit BRCC36, BRCA1/BRCA2-containing Complex Subunit 3, BRCA1/BRCA2-containing Complex Subunit 36, BRISC Complex Subunit BRCC36, BRCC3, C6.1A, CXorf53)
Description:
BRCC-36 is a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. It is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, and does not have activity toward 'Lys-48'-linked polyubiquitin chains. In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to 18aa from human BRCC36 at N-terminal (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity:
Recognizes human BRCC36. Species sequence homology: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.