		Technical Data
B2727-01D	BRCC-36 (BRCA1/BRCA2-containing Complex Subunit 36, BRCC36, BRCA1/BRCA2-containing Complex Subunit 3, BRCC3, C6.1A, CXorf53, RP11-143H17.2)

Description:
BRCC-36 is a subunit of the BRCA1-BRCA2-containing complex (BRCC), which is an E3 ubiquitin ligase. It is a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains, and does not have activity toward 'Lys-48'-linked polyubiquitin chains. In the BRCA1-A complex, it specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX, antagonizing the RNF8-dependent ubiquitination at double-strand breaks (DSBs). This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint (1).

Applications:
Suitable for use in Flow Cytometry, Western Blot, and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100-1:500
Western Blot: 1:1000-1:50,000
Immunoprecipitation: 1:10-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab		10J91	Supernatant

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

Not determined

Immunogen:

Synthetic peptide corresponding to residues in human BRCC-36.

Purity:

Supernatant

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.

Specificity:

Recognizes human BRCC-36 at ~36kD. Species Crossreactivity: mouse, rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8.