Technical Data
Breast Cancer Resistance Protein (BCRP)
Breast Cancer Resistance Protein (BCRP) is a 70kD ATP-Binding Cassette membrane transport protein involved in multidrug resistance. BCRP may be over-expressed in cancer cell lines selected with doxorubicin / verapamil, topotecan or mitoxantrone.

Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): 1:20 dilution on acetone fixed frozen sections followed by incubation with rabbit anti-mouse IgG and a monoclonal mouse APAAP complex.
Immunohistochemistry (Paraffin): Use on formaldehyde-fixed paraffin-embedded human tissues and tumors after pretreatment with 10mM citric acid, pH 6.0, for 20 min.
Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
125ug-20°CBlue IceHumanMouse
Not determined
Recombinant protein corresponding to aa271-396 from human Breast Cancer Resistance Protein.
Supplied as a liquid in 0.7% BSA, 0.09% sodium azide.
Recognizes an internal epitope of human Breast Cancer Resistance Protein. Does not cross-react with human P-glycoprotein, MRP1 or MRP2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. M. Maliepaard, et al.; Cancer Res. 61, 3458 (2001) 2. C. Özvegy, et al.; J. Biol. Chem. 277, 47980 (2002)