Technical Data
Brefeldin A (g-4-Dihdyroxy-2-(6-hydroxy-1-heptenyl)-4-cyclopentanecrotonic Acid, -Lactone, BFA, Cyanein, Ascotoxin, Decumbin, Synergisidin, Nectrolide)
Biochemicals Storage: -20CShipping: Blue Ice
Brefeldin A (BFA) is a fungal metabolite demonstrated to reversibly interfere with anterograde transport from the endoplasmic reticulumto the Golgi apparatus (1,2). While initially isolated as an antibiotic (3), and does have a wide range of antibiotic activity, it is primarily used as a biological research tool for studying protein transport. Treatment leads to a rapid accumulation of proteins within the ER and collapse of the Golgi stacks. Treatment with BFA can also inhibit protein secretion (4) and prolonged exposure can induce apoptosis (5). The main target of BFA appears to be ADP-ribosylation factor (ARF), which is responsible for association of coat protein to the Golgi membrane (6,7).

Suitable for use in Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunofluorescence: 100ng/ml-10ug/ml. Inhibiton of ER to Golgi trafficking was observed as low as 100ng/ml and apoptosis was observed with prolonged treatment at 10ug/ml.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with DMSO or ethanol. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

CAS Number:

Molecular Formula:

Molecular Weight:
Form: Supplied as a lyophillized powder. Reconstitute with DMSO, ethanol or methanol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Klausner, R.D. et al. (1992) J Cell Biol 116, 107180. 2. Pelham, H.R. (1991) Cell 67, 44951. 3. Tamura, G. et al. (1968) J Antibiot (Tokyo) 21, 1601. 4. Misumi, Y. et al. (1986) J Biol Chem 261, 11398403. 5. Shao, R.G. et al. (1996) Exp Cell Res 227, 1906. 6. Helms, J.B. and Rothman, J.E. (1992) Nature 360, 3524. 7. Randazzo, P.A. et al. (1993) J Biol Chem 268, 955563.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.