Technical Data
Media
Brilliant Green Agar (Powder)
B2750
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Casein Digest Peptone 5.0
Lactose10.0
Yeast Extract3.0
Brilliant Green0.0125
Peptic Digest of Animal Tissue5.0
Sodium Chloride5.0
Sucrose10.0
Phenol Red0.8
Agar20.0
Total:58g/liter
Selective medium for isolation of Salmonella.

Description:
Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for typhoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: 1. Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige with a greenish tint, homogeneous, free flowing powder.

Solubility: Brownish-green, slightly turbid.

pH (25C): 6.90.2

Microbiological Analysis: Escherichia coli ATCC 25922


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.