Technical Data

Media

Brilliant Green Agar (Powder)

B2750

Microbiological Grade

Storage RT/4°C Shipping RT

Components shown as g/liter


Casein Digest Peptone	5.0
Lactose	10.0
Yeast Extract	3.0
Brilliant Green	0.0125
Peptic Digest of Animal Tissue	5.0
Sodium Chloride	5.0
Sucrose	10.0
Phenol Red	0.8
Agar	20.0

Total:	58g/liter

Selective medium for isolation of Salmonella.

Description:
Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for typhoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Directions: 1. Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: Light beige with a greenish tint, homogeneous, free flowing powder.

Solubility: Brownish-green, slightly turbid.

pH (25°C): 6.9±0.2

Microbiological Analysis: Escherichia coli ATCC 25922

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.