Technical Data
Media
Brilliant Green Bile Broth 2% (Powder)
B2750-06
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Pancreatic Digest of10.0
Lactose10.0
Oxbile20.0
Brilliant Green0.0133
Total:40g/liter
Brilliant Green 2% Bile Broth is used to confirm the presence of coliforms. Oxbile and Brilliant Green inhibit Gram positive bacteria and many Gram negative bacteria other than coliforms. Lactose is a carbohydrate source. Bacteria that ferment lactose and produce gas are detected. This medium is formulated according to AOAC specifications.

Storage and Stability:
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.


Directions: 1. Dissolve 40grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light greenish beige, homogeneous, free flowing powder. Solubility

pH (4% at 25C): 7.20.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.